Molecular Microbiology Research LaboratoryLokender Kumar Ph.D / Research Assistant Professor / Department of Physics
Contact for Undergraduate/Senior Design Research Project Positions
Research Area : Molecular Microbiology
Antimicrobial resistance is developing and spreading worldwide, threatening our capability to cure common infectious diseases. My research interests focus on understanding antibiotic resistance and finding alternate antimicrobial strategies against opportunistic human pathogens including Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Enterococcus faecalis/faecium. My research interests center on developing antivirulence therapy and bacteriophage therapy to treat multi drug resistant pathogen infections. My lab wants to understand the mechanism of intra/interspecies signaling systems in bacteria that involve the exchange of chemical signals (autoinducers), linking the regulation of virulence gene expression with population density. Quorum sensing system is best target to develop antivirulence drug therapy. This therapy include the use of compounds that inhibit or neutralize the effect/function of the virulence factors. Second dimension of my research is the use of viruses to kill drug resistance bacteria. Bacteriophages (phages) are described as viruses that infect bacteria and found to be effective against antibiotic resistant bacteria. Lytic phages infect a host bacterium and use host machinery to replicate leading to host cell lysis and death. Lytic phages (virulent phages) belong to the class of natural antimicrobial agents. The ultimate goal of my research is to exploit quorum sensing system and bacteriophage therapy to develop bio-detection assays, anti-virulence therapies, and effective phage therapy against multi drug resistant bacterial pathogens.
Lokender Kumar, John Brice, Linda Toberer ,Judith Klein-Seetharaman, Daniel Knauss, Susanta Kumar Sarkar. Antimicrobial biopolymer formation from sodium alginate and algae extract using aminoglycosides. PLoS ONE (2019) 14(3): e0214411.
Lokender Kumar, Christopher Cox and Susanta Kumar Sarkar. Matrix metalloprotease-1 inhibits and disrupts Enterococcus faecalis PLoS ONE (2019) 14(1): e0210218.
Lokender Kumar, Warren Columb, John Czerski, Christopher cox and Susanta Kumar Sarkar. Efficient protease based purification of recombinant matrix metalloproteinase-1 in coli. Protein expression and purification. (2018) 148: 59-67.
Lokender Kumar, Sanjay Chhibber, Rajnish Kumar, Manoj Kumar and Kusum Harjai. Zingerone silences quorum sensing and attenuates virulence of Pseudomonas aeruginosa. Fitoterapia (2015) 102:84-95. For the first time our research proved zingerone as anti-virulence drug against Pseudomonas aeruginosa
Lokender Kumar, Sanjay Chhibber and Kusum Harjai. Zingerone suppresses liver inflammation induced by antibiotic mediated endotoxemia through down regulating hepatic mRNA expression of inflammatory markers in Pseudomonas aeruginosa peritonitis mouse model. PLoS ONE (2014) 9(9): e106536. Research was selected in The International Liver Congress™ 2014 – 49th Annual meeting of the European Association for the Study of the Liver. 9-13 April 2014 • London, United Kingdom.
Lokender Kumar, Sanjay Chhibber and Kusum Harjai. Structural alterations in Pseudomonas aeruginosa by zingerone contribute to enhanced susceptibility to antibiotics, serum and phagocytes. Life Sciences (2014) 117(1):24-32.
Lokender Kumar, Sanjay Chhibber and Kusum Harjai. Zingerone inhibit biofilm formation and improve antibiofilm efficacy of ciprofloxacin againstPseudomonas aeruginosa Fitoterapia (2013) 90:73-78
Lokender Kumar, Ravi Kumar Gupta, Sanjay Chhibber and Kusum Harjai. Optimization of growth conditions and purification of quorum sensing signal molecules produced by Pseudomonas aeruginosa. International Journal of Pharma and Bio Sciences (2013) 4(1):507-514.
Lokender Kumar, Sanjay Chhibber and Kusum Harjai. Hepatoprotective effect of zingerone [4-(4- hydroxy-3-methoxyphenyl) butan-2-one] in lipopolysaccharide induced liver injury mouse model through downregulation of inflammatory mediators. International Journal of Pharmacognosy and Phytochemical Research, (2014) 6(2): 308-316
Lokender Kumar, Kusum Harjai and Sanjay Chhibber. Recent Update on Multiple Pharmacological Benefits of Zingerone: A Quick Review, American Journal of Phytomedicine and Clinical Therapeutics, (2014) 2(6):693-704 (Invited Review).
Anju Bala, Lokender Kumar, Sanjay Chhibber, Kusum Harjai. Augmentation of virulence related traits of pqs mutants by Pseudomonas quinolone signal through membrane vesicles. Journal of Basic Microbiology (2015) 55:566-578. (Selected as the cover page image for Journal of Basic Microbiology).
Novel protease and mild detergent based one step purification of active MMP1 protein in E.coli. US Provisional Application no. US-4843-157.
Anti-biofilm activity of MMP1 against Enterococcus faecalis (US Patent Filed)
Antimicrobial biopolymer from sea weed (US Patent Filed)
Post Doc. Single Molecule Biophysics Laboratory, Colorado School of Mines (CSM)
Post Doc. Immunology Laboratory, Institute of Microbial Technology (IMTECH) Ph.D. (Microbiology)
Ph.D. Department of Microbiology, Panjab University (PU) M. Sc. (Microbiology) Himachal Pradesh University (HPU)
Gold Medal (M.Sc. Microbiology), Himachal Pradesh University (HPU)
INSPIRE Fellow, Department of Science and Technology (DST)
UGC-CSIR NET Fellow, Council of Scientific and Industrial Research (CSIR)
Best poster award in Engineering Physics Senior Design Colloquium, CSM, USA).
Synthetic Bacteriophages: Applications in detection of bacteria and antibacterial bacteriophage therapy
Antiquorum sensing activity of beta-lactam antibiotics
Mechanism of persister cell evolution in Pseudomonas aeruginosa